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KMID : 0620919980300010021
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Experimental & Molecular Medicine
1998 Volume.30 No. 1 p.21 ~ p.27
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Changes of phospholipase D activity in TNF-α and anti-Fas/Apo1 monoclonal antibody induced apoptosis in HL-60 and A20 cells
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Joong Soo Han/Jin Ho Kang
In Cheol Shin/Joong Soo Han
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Abstract
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The changes of phospholipase D (PLD) activity were investigated during the courses of apoptotic process induced by tumor necrosis factor (TNF)-α or anti-Fas/Apo1 antibody in human premyelocyte HL-60 and murine B cell lymphoma A20 cells. The treatment of recombinant TNF-α to HL-60 cells resulted in the increased PLD activity as determined by the phosphatidylethanol formation in the presence of 1% ethanol. The enhancement of PLD activity was also observed in the anti-Fas/Apo1 monoclonal antibody-treated A20 cells. However, the activity of PLD was maximized when HL-60 and A20 cells were treated with either TNF-α or anti-Fas/Apo1 monoclonal antibody for 6 h. Both TNF-α and anti-Fas/Apo1 monoclonal antibody increased PLD activity in a dose-dependent manner up to 200 U/ml and 200 ng/ml, respectively. When the intracellular activity of protein kinase C (PKC) was interrupted by treatment of calphostin-C, both the PLD activation and the apoptosis induced by TNF-α and anti-Fas/Apo1 monoclonal antibody appeared to be inhibited. Since PKC is reported to activate PLD, the results indicate that the intracellular signaling cascade via PLD may play a role in the induction of apoptosis induced by TNF-α and anti-Fas/Apo1 monoclonal antibody.
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KEYWORD
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apoptosis, anti-Fas monoclonal antibody, phospholipase D, protein kinase C, tumor necrosis factor-α,
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